Education

B.S. 2008, East China University of Science and Technology, Biotechnology;
Ph.D. 2013, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Biochemistry and Molecular Biology;

Professional Experience

Postdoctoral Fellow, Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, 2013-2014;
Postdoctoral Fellow, KU Leuven, 2014-2016;
Postdoctoral Fellow/Senior Scientist, Department of Immunology, St. Jude Children’s Research Hospital, 2017-2021;
Professor, School of Life Sciences, Xiamen University, 2022 to Present.

Research Area

Our research interest lies in the immune signaling underlying the differentiation and function of T cells, a central cell type in adaptive immunity and cancer immunotherapy. We apply systems biology approaches, such as single cell RNA-sequencing (scRNA-seq), CRISPR screening and the integrative network analysis, to reconstruct molecular hubs for T cell fate decision in tumor, infection, and autoimmunity diseases as well as in heathy condition. Our work could shed light on our understanding of fundamental mechanisms of immune regulation and pave the way for better immunotherapy.

Selected Publications

1.Shengjun Geng1, #, Zifeng Li1, #, Wenhao Li1, Huiqing Liang1, Fan Xu1, Leilei Zhang1, Wei Zhang1, Shengyong Tao1, Yanhan Wang1, Yurui Zhou2, Shijie Luo3, Xing Lei4, Hailang Li5, Wei-Feng Huang6, Meixia Che2, Wen-Hsien Liu7, Jialiang Huang3,7, Tianying Zhang4, Kejia Wang8, Nengming Xiao7, Kairui Mao1, Yajun Jiang2 and Hongling Huang1, 9, * (2026) Krüppel-like factor 2 programs early exhausted T cell states and restrains antiviral immunity, Immunity (In press).

2.Zhou, P#., H. Shi#, H. Huang#, X. Sun, S. Yuan, N. M. Chapman, J. P. Connelly, S. A. Lim, J. Saravia, A. Kc, S. M. Pruett-Miller and H. Chi* (2023). "Single-cell CRISPR screens in vivo map T cell fate regulomes in cancer." Nature. 624, pages154–163 (2023).
3.Guo, A.#, H. Huang#, Z. Zhu, M. J. Chen, H. Shi, S. Yuan, P. Sharma, J. P. Connelly, S. Liedmann, Y. Dhungana, Z. Li, D. Haydar, M. Yang, H. Beere, J. T. Yustein, C. DeRenzo, S. M. Pruett-Miller, J. C. Crawford, G. Krenciute, C. W. M. Roberts, H. Chi* and D. R. Green* (2022). "cBAF complex components and MYC cooperate early in CD8(+) T cell fate." Nature 607(7917): 135-141.
4.Huang, H.#, P. Zhou, J#. Wei, L. Long, H. Shi, Y. Dhungana, N. M. Chapman, G. Fu, J. Saravia, J. L. Raynor, S. Liu, G. Palacios, Y. D. Wang, C. Qian, J. Yu and H. Chi *(2021). "In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8(+) T cell fate decisions." Cell 184(5): 1245-1261 e1221.
5.Huang, H#., L. Long, P#. Zhou, N#. M. Chapman and H. Chi* (2020). "mTOR signaling at the crossroads of environmental signals and T-cell fate decisions." Immunol Rev 295(1): 15-38.
6.Huang, H., S. Vandekeere, J. Kalucka, L. Bierhansl, A. Zecchin, U. Bruning, A. Visnagri, N. Yuldasheva, J. Goveia, B. Cruys, K. Brepoels, S. Wyns, S. Rayport, B. Ghesquiere, S. Vinckier, L. Schoonjans, R. Cubbon, M. Dewerchin, G. Eelen and P. Carmeliet (2017). "Role of glutamine and interlinked asparagine metabolism in vessel formation." Embo j 36(16): 2334-2352.
7.Huang, H. L., S. Wang, M. X. Yin, L. Dong, C. Wang, W. Wu, Y. Lu, M. Feng, C. Dai, X. Guo, L. Li, B. Zhao, Z. Zhou, H. Ji, J. Jiang, Y. Zhao, X. Y. Liu and L. Zhang (2013). "Par-1 regulates tissue growth by influencing hippo phosphorylation status and hippo-salvador association." PLoS Biol 11(8): e1001620.
8.Su, W., J. Saravia, I. Risch, S. Rankin, C. Guy, N. M. Chapman, H. Shi, Y. Sun, A. Kc, W. Li, H. Huang, S. A. Lim, H. Hu, Y. Wang, D. Liu, Y. Jiao, P. C. Chen, H. Soliman, K. K. Yan, J. Zhang, P. Vogel, X. Liu, G. E. Serrano, T. G. Beach, J. Yu, J. Peng and H. Chi* (2023). "CXCR6 orchestrates brain CD8(+) T cell residency and limits mouse Alzheimer's disease pathology." Nat Immunol 24(10): 1735-1747.
9.Long, L., J. Wei, S. A. Lim, J. L. Raynor, H. Shi, J. P. Connelly, H. Wang, C. Guy, B. Xie, N. M. Chapman, G. Fu, Y. Wang, H. Huang, W. Su, J. Saravia, I. Risch, Y. D. Wang, Y. Li, M. Niu, Y. Dhungana, A. Kc, P. Zhou, P. Vogel, J. Yu, S. M. Pruett-Miller, J. Peng and H. Chi* (2021). "CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity." Nature 600(7888): 308-313.
10.Fu, G., C. S. Guy, N. M. Chapman, G. Palacios, J. Wei, P. Zhou, L. Long, Y. D. Wang, C. Qian, Y. Dhungana, H. Huang, A. Kc, H. Shi, S. Rankin, S. A. Brown, A. Johnson, R. Wakefield, C. G. Robinson, X. Liu, A. Sheyn, J. Yu, S. Jackowski and H. Chi*(2021). "Metabolic control of TFH cells and humoral immunity by phosphatidylethanolamine." Nature 595(7869): 724-729.
11.Wei, J., L. Long, W. Zheng, Y. Dhungana, S. A. Lim, C. Guy, Y. Wang, Y. D. Wang, C. Qian, B. Xu, A. Kc, J. Saravia, H. Huang, J. Yu, J. G. Doench, T. L. Geiger and H. Chi* (2019). "Targeting REGNASE-1 programs long-lived effector T cells for cancer therapy." Nature 576(7787): 471-476.
12.Eelen, G., C. Dubois, A. R. Cantelmo, J. Goveia, U. Bruning, M. DeRan, G. Jarugumilli, J. van Rijssel, G. Saladino, F. Comitani, A. Zecchin, S. Rocha, R. Chen, H. Huang, S. Vandekeere, J. Kalucka, C. Lange, F. Morales-Rodriguez, B. Cruys, L. Treps, L. Ramer, S. Vinckier, K. Brepoels, S. Wyns, J. Souffreau, L. Schoonjans, W. H. Lamers, Y. Wu, J. Haustraete, J. Hofkens, S. Liekens, R. Cubbon, B. Ghesquiere, M. Dewerchin, F. L. Gervasio, X. Li, J. D. van Buul, X. Wu and P. Carmeliet* (2018). "Role of glutamine synthetase in angiogenesis beyond glutamine synthesis." Nature 561(7721): 63-69.
13.Wong, B. W., X. Wang, A. Zecchin, B. Thienpont, I. Cornelissen, J. Kalucka, M. Garcia-Caballero, R. Missiaen, H. Huang, U. Bruning, S. Blacher, S. Vinckier, J. Goveia, M. Knobloch, H. Zhao, C. Dierkes, C. Shi, R. Hagerling, V. Moral-Darde, S. Wyns, M. Lippens, S. Jessberger, S. M. Fendt, A. Luttun, A. Noel, F. Kiefer, B. Ghesquiere, L. Moons, L. Schoonjans, M. Dewerchin, G. Eelen, D. Lambrechts and P. Carmeliet* (2017). "The role of fatty acid beta-oxidation in lymphangiogenesis." Nature 542(7639): 49-54.