Education

B.S. 2012, Xiamen University, Biology;
Ph.D. 2017, Tsinghua University, Biology;

Professional Experience

Postdoctoral Fellow/Associate Professor, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, 2017-2021;
Professor, School of Life Sciences, Xiamen University, 2022 to Present.

Research Area

Synthetic genomics is an important part of synthetic biology, which is expected to innovate our understanding about genome function and to significantly improve the depth and ability of genome engineering. We participated in the world's first eukaryotic genome synthesis project, constructed the longest eukaryotic linear synthetic chromosome so far, identified the Writers and Erasers of histone 2-hydroxyisobutyrylation, and created technologies for synthetic chromosome design, construction and application. Our results have been published in journals such as Science (cover story and special issue), Cell Res, Nat Commun and Genome Biol. In the future, our research group will continue to focus on the basic theories, key technologies and new applications related to genome.

Selected Publications (*Corresponding author, #co-first author)

1.W. Zhang^*, G. Zhao^*, Z. Luo^*, Y. Lin, L. Wang, Y. Guo, A. Wang, S. Jiang, Q. Jiang, J. Gong, Y. Wang, S. Hou, J. Huang, T. Li, Y. Qin, J. Dong, Q. Qin, J. Zhang, X. Zou, X. He, L. Zhao, Y. Xiao, M. Xu, E. Cheng, N. Huang, T. Zhou, Y. Shen, R. Walker, Y. Luo, Z. Kuang, L.A. Mitchell, K. Yang, S.M. Richardson, Y. Wu, B.-Z. Li, Y.-J. Yuan, H. Yang, J. Lin, G.-Q. Chen, Q. Wu, J.S. Bader, Y. Cai, J.D. Boeke, J. Dai^#, Engineering the ribosomal DNA in a megabase synthetic chromosome, Science.355 (2017) eaaf3981.
2.Y. Shen^*, Y. Wang^*, T. Chen^*, F. Gao, J. Gong, D. Abramczyk, R. Walker, H. Zhao, S. Chen, W. Liu, Y. Luo, C.A. Müller, A. Paul-Dubois-Taine, B. Alver, G. Stracquadanio, L.A. Mitchell,Z. Luo, Y. Fan, B. Zhou, B. Wen, F. Tan, Y. Wang, J. Zi, Z. Xie, B. Li, K. Yang, S.M. Richardson, H. Jiang, C.E. French, C.A. Nieduszynski, R. Koszul, A.L. Marston, Y. Yuan, J. Wang, J.S. Bader, J. Dai, J.D. Boeke, X. Xu, Y. Cai^#, H. Yang^#, Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome, Science. 355 (2017) eaaf4791.
3.G. Mercy^*, J. Mozziconacci^*, V.F. Scolari, K. Yang, G. Zhao, A. Thierry, Y. Luo, L.A. Mitchell, M. Shen, Y. Shen, R. Walker, W. Zhang, Y. Wu, Z. Xie,Z. Luo, Y. Cai, J. Dai, H. Yang, Y.-J. Yuan, J.D. Boeke, J.S. Bader, H. Muller^#, R. Koszul^#, 3D organization of synthetic and scrambled chromosomes, Science. 355 (2017) eaaf4597.
4.Z. Luo^*, L. Wang^*, Y. Wang, W. Zhang, Y. Guo, Y. Shen, L. Jiang, Q. Wu, C. Zhang, Y. Cai^#, J. Dai^#, Identifying and characterizing SCRaMbLEd synthetic yeast using ReSCuES, Nat. Commun.9 (2018) 1930.
5.W. Liu^*,Z. Luo, Y. Wang, N.T. Pham, L. Tuck, I. Pérez-Pi, L. Liu, Y. Shen, C. French, M. Auer, J. Marles-Wright, J. Dai^#, Y. Cai^#, Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods, Nat. Commun.9 (2018) 1936.
6.Z. Luo^#*, K. Yu^*, S. Xie, M. Monti, D. Schindler, Y. Fang, S. Zhao, Z. Liang, S. Jiang, M. Luan, C. Xiao, Y. Cai^#, J. Dai^#, Compacting a synthetic yeast chromosome arm, Genome Biol.22 (2021) 1-18.
7.J. Huang^*, Z. Luo^*, W. Ying^*, Q. Cao, H. Huang, J. Dong, Q. Wu, Y. Zhao, X. Qian^#, J. Dai^#, 2-Hydroxyisobutyrylation on histone H4K8 is regulated by glucose homeostasis in Saccharomyces cerevisiae, Proc. Natl. Acad. Sci. 114 (2017) 8782–8787.
8.H. Huang^*, Z. Luo^*, S. Qi^*, J. Huang^*, P. Xu, X. Wang, L. Gao, F. Li, J. Wang, W. Zhao, W. Gu, Z. Chen, L. Dai^#, J. Dai^#, Y. Zhao^#, Landscape of the regulatory elements for lysine 2-hydroxyisobutyrylation pathway, Cell Res. 28 (2018) 111–125.
9.Y. Wang^*, W. Li^*, A.E. Johnson^*, Z. Luo^*, X. Sun, A. Feoktistova, W.H. McDonald, I. McLeod, J.R. Yates, K.L. Gould, D. McCollum^#, Q. Jin^#, Dnt1 acts as a mitotic inhibitor of the spindle checkpoint protein dma1 in fission yeast, Mol. Biol. Cell. 23 (2012) 3348–3356.
10.Z. Luo, J. Dai, Synthetic genomics: the art of design and synthesis, Chin. J. Biotechnol. 33 (2017) 331–342.
11.Z. Luo, Q. Yang, B. Geng, S. Jiang, S. Yang, X. Li, Y. Cai, J. Dai^#, Whole genome engineering by synthesis, Sci. China Life Sci. (2018).1515–1527.
12.J. Lu, Z. Luo, S. Jiang, Y. Shen, Y. Wu, H. Yang, Y. Yuan, J. Dai^#, Technologies for DNA Synthesis, Assembly, and Transplantation. Bulletin of Chinese Academy of Sciences, 33(2018) 1174-1183.
13.B. Li, Y. Wu, Z. Xie, Y. Shen, Y. Wang, W. Zhang, G. Zhao, Z. Luo, J. Dai, H. Yang, Y. Yuan^#, Precise Synthesis of Designer Large Chromosomes of Saccharomyces cerevisiae. Frontier Science, 12(2018) 55-59.
14.Z. Luo, S. Hoffmann, S. Jiang, Y. Cai^#, J. Dai^#. Probing eukaryotic genome functions with synthetic chromosomes. Exp Cell Res. 390(2020) 111936.
15.J. Dai, J.D. Boeke, Z. Luo, S. Jiang, Y. Cai^#, Sc3.0: revamping and minimizing the yeast genome, Genome Biol. 21(2020) 1-4.
16.Z. Luo, S. Jiang, J. Dai, Chromosomal Rearrangements of Synthetic Yeast by SCRaMbLE, Methods Mol Biol. 2021;2196:153-165.
17.S. Jiang, Z. Luo, J. Dai. Use YeastFab to Construct Genetic Parts and Multicomponent Pathways for Metabolic Engineering, Methods Mol Biol. 2021;2196:167-180.