Education

1988, B.Sc., Wuhan University;

1991, M.Sc., Fuzhou University;

1994, Ph.D., Xiamen University.

Professional Experience

1994-1996, Lecturer, Department of Biology, Xiamen University;

1996-1997, Visiting Scholar at Prof. Masoko Maeda's laboratory, School of Pharmaceutical Sciences, Showa University, Tokyo, Japan, Visiting Scholar in Prof. Totaro Imasaka's laboratory, Faculty of Engineering, Kyushu University, Fukuoka, Japan;

1996-2002, Associate Professor, The Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University;

2000-2002, Visiting Investigator, Public Health Research Institute, New York, USA;

2002-Present, Professor, School of Life Sciences, Xiamen University; 

2009-Present: Director of Engineering Research Centre of Molecular Diagnostics, Ministry of Education, School of Life Sciences, Xiamen University.

Research Area

Our group is committed to the development and translation of novel techniques, biomarkers and their combination into molecular diagnosis that is applicable in clinical settings. We invented displacement probes, a new class of probes for real-time PCR that have wide applications in gene quantification and genotyping. We developed silica-based fluorescent lanthanide chelate-coated nanoparticles, which have been used as a new generation of label for lateral flow immunoassays. Recently, we developed multicolor melting curve analysis (MMCA), which could be a universal medium-plex and high throughput approach for sequence variant detection. We are now exploring MMCA in the diagnosis of variety of diseases such as cancers, inherited diseases, infectious diseases and pharmacogenomics, etc. By taking advantages of the rapid evolution of genomics study, we will continue our work to improve the current status of the analytical tool in human diseases.

Selected Publications

1. Chen Du^#, Yiqun Liao^#, Congcong Ding, Jiayu Huang, Shujuan Zhou, Yiyan Xu, Zhaohui Yang, Xiaolu Shi, Yinghui Li, Min Jiang, Le Zuo, Minxu Li, Shengzhe Bian, Na Xiao, Liqiang Li, Ye Xu^*, Qinghua Hu^*, Qingge Li^*, Molecular serotyping of diarrheagenic Escherichia coli with a MeltArray assay reveals distinct correlation between serotype and pathotype, Gut Microbes, 16(1):2401944, 2024.（IF=12.2004）
2. Qiuying Huang^#, Dongmei Chen^#, Chen Du^#, Qiaoqiao Liu, Su Lin, Lanlan Liang, Ye Xu, Yiqun Liao^*, Qingge Li^*, Highly multiplex PCR assays by coupling the 5-flap endonuclease activity of Taq DNA polymerase and molecular beacon reporters, PNAS, 119(9): e2110672119, 2022.（IF=11.205）
3. Xinwen Chen, Yifan Li, Qiuying Huang, Xingming Lin, Xudong Wang, Yafang Wang, Ying Liu, Qiushun He, Yinghua Liu, Ting Wang^*, Zhi-Liang Ji^*, Qingge Li^*, Segmental duplication as potential biomarkers for non-invasive prenatal testing of aneuploidies, EBioMedicine, 70:103535, 2021.（IF=9.7004）
4. Yiqun Liao, Xiaobo Wang, Chao Sha, Zhongmin Xia, Qiuying Huang^*, Qingge Li^*, Combination of fluorescence color and melting temperature as a two-dimensional label for homogeneous multiplex PCR detection, Nucleic acids research, 41(7):e76, 2013.（IF=16.6009）
5. Qiwei Guo^#, Yulin Zhou^#, Xiaobo Wang, Qingge Li^*, Simultaneous detection of trisomies 13, 18, and 21 with multiplex ligation- dependent probe amplification-based real-time PCR, Clinical Chemistry, 56(9):1451-1459, 2010.（IF=7.1）
6. Ying Liu^#, Yinghua Liu^#, Liu Guo, Yazhe Wu, Yafang Wang, Lingzhen Xu, Mingzhu Xu, Siyu Huang, Ping Chen, Ting Wang, Qiuying Huang^*, Qingge Li^*, Multiplex Asymmetric PCR by Combining the Amplification Refractory Mutation System with the Homo-Tag-Assisted Nondimer System, Analytical Chemistry, 96(22):9200-9208, 2024.（IF=6.7）
7. Zihan Xia^#, Biyi Su^#, Chunxia Tu^#, Siyang Sun, Yaoju Tan, Ye Xu^*, Qingge Li^*, Single-tube protocol for culture-independent spoligotyping of Mycobacterium tuberculosis based on MeltArray, Journal of Clinical Microbiology, 62(1): e0118323, 2024.（IF=6.1004）
8. Siqi Zhang^#, Xiaohong Chen^#, Zhonghui Lin^#, Yaoju Tan, Bin Liang, Yuying Pan, Mingxiang Huang, Biyi Su, Xiaoman Hu, Ye Xu^*, Qingge Li^*,Quantification of Isoniazid-Heteroresistant Mycobacterium tuberculosis Using Droplet Digital PCR, Journal of Clinical Microbiology, 61(6):1-12, 2023.（IF=6.1004）
9. Qiuying Huang, Qinghua Hu, Qingge Li^*, Identification of eight foodborne pathogens by multicolor combinational probe coding technology in a single real-time PCR, Clinical Chemistry, 53(10):1741–1748, 2007.（IF=8.327)
10. Jinping Cheng, Yongyou Zhang, Qingge Li^*, Real-time PCR genotyping using displacing probes, Nucleic Acids Research, 32(7): E61, 2004.（IF=16.6009)