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李庆阁教授博导

发布时间:2015-11-27来源:点击数:11825

 

李庆阁教授李庆阁  LI Qingge, Ph.D.   

教授,博士生导师

分子诊断学Molecular Diagnostics

课题组组长, 分子诊断教育部工程研究中心负责人

中心网站:http://md.xmu.edu.cn

  话:+86-592-2182100

E-mailqgli@xmu.edu.cn

 

武汉大学化学系分析化学专业本科学士(1988),

福州大学化学系分析化学专业硕士(1991),

厦门大学化学系分析化学专业理学博士(1994),

1996年至1997年,日本昭和大学药学部和日本九州大学工学部访问学者,

2000年至2002年美国公共卫生研究所和日本早稻田大学工学部访问学者,

1994年至今,厦门大学生命科学学院,讲师(1994),副教授(1996),

教授(2002),博士生导师(2004),分子诊断教育部工程研究中心主任(2009)。

 

研究领域(Research Area)

本课题组一直从事新技术的研发和疾病标志物的发现,并将二者结合起来转化为临床应用的分子诊断。我们发明了置换探针,该探针作为实时PCR的一种新型探针已广泛用于基因定量和突变检测。我们还研发了一种硅基荧光稀土络合物纳米颗粒,现已成为免疫层析检测的新一代通用标记物。近来,我们提出了多色熔解曲线分析技术(MMCA),该技术有望成为核酸变异的中通量分析工具,目前,我们正将之用于肿瘤、遗传病、传染病和药物基因组学等相关疾病的诊断。伴随着基因组研究的快速发展,本课题组将继续致力于推动人类疾病诊断技术的进步。

Our group is committed to the development and translation of novel techniques, biomarkers and their combination into molecular diagnosis that is applicable in clinical settings. We invented displacement probes, a new class of probes for real-time PCR that have wide applications in gene quantification and genotyping. We developed silica-based fluorescent lanthanide chelate-coated nanoparticles, which have been used as a new generation of label for lateral flow immunoassays. Recently, we developed multicolor melting curve analysis (MMCA), which could be a universal medium-plex and high throughput approach for sequence variant detection. We are now exploring MMCA in the diagnosis of variety of diseases such as cancers, inherited diseases, infectious diseases and pharmacogenomics, etc. By taking advantages of the rapid evolution of genomics study, we will continue our work to improve the current status of the analytical tool in human diseases.

 

代表性论文(Selected Publications, *Corresponding author)

1. Heng Zhang, Ye Xu, Qiuying Huang, Changqing Yi, Tan Xiao and Qingge Li*, Natural phage nanoparticle-mediated real-time immuno-PCR for ultrasensitive detection of protein marker, Chemical Communications, 49(36):3778-80, 2013. IF=6.378)
 2.Yiqun Liao, Xiaobo Wang, Chao Sha, Zhongmin Xia, Qiuying Huang* and Qingge Li,* Combination of fluorescence color and melting temperature as a two-dimensional label for homogeneous multiplex PCR detection, Nucleic acids research, 41(7):1-7, 2013. IF=8.278)
 3.Fu Xiong, Qiuying Huang, Xiaoyun Chen, Yanxia Qu, Qingge Li* and Xiangmin Xu*, A Melting Curve AnalysisBased PCR Assay for One-Step Genotyping of β-Thalassemia Mutations :A Multicenter Validation, Journal of Molecular Diagnostics, 13(4):427-435, 2011. IF=3.952)
4.Guo Q, Zhou Y, Wang X, Li Q*,Simultaneous Detection of Trisomies 13, 18, and 21 with Multiplex Ligation-Dependent Probe Amplification- Based Real-Time PCR, Clinical Chemistry, 56(9) :1451-1459, 2010. IF=7.149)
 5.Xiaohu Xia, Ye Xu, Qingge Li*,Lateral flow immunoassay using europium chelate-loaded silica nanoparticles as labels, Clinical Chemistry, 55(1) :179-182, 2009. IF=7.149)
6. Heng Zhang, Ye Xu, Wei Yang, Qingge Li*, Dual-Lanthanide-Chelated Silica Nanoparticles as Labels for Highly Sensitive Time-resolved Fluorometry, Chemistry of Materials,19: 5875–5881, 2007.IF=8.238)
7.Qiuying Huang, Qinghua Hu, Qingge Li*, Identification of eight foodborne pathogens by multicolor combinational probe coding technology in a single real-time PCR, Clinical Chemistry, 53(10): 1741–1748, 2007. IF=7.149)
8.Ye Xu, Qingge Li*, Multiple Fluorescent labeling of silica nanoparticles with lanthanide chelates for highly sensitive time-resolved immunofluorometric assays, Clinical Chemistry, 53(8):1503–1510, 2007.IF=7.149)